use | a biotinylation reagent incorporating an aminocaproyl holmium pacer' this can reduce the steric hindrance in binding avidin to some biotinylated compounds when used to biotinylate both the antibody and th e carrier in the Protein Avidin-Biotin Capture system. this system avoids the direct interaction of a captured antibody with solid surfaces, such as plastics, which may reduce antigenicity. It has a lso been used to enhance the detection of DNA on nitrocellulose. has been used as a cell surface labeling reagent |